Eppendorf Micromanipulator 5171: Features, Specifications and Applications
Eppendorf Micromanipulator 5171 Manual: Everything You Need to Know
If you are looking for a device that can help you perform microinjection and cell manipulation with ease and precision, you might want to consider the Eppendorf Micromanipulator 5171. This device is designed to combine the classic features of a mechanical system with the advantages of an electromotive system, offering optimal functionality and simple operation. In this article, we will provide you with a comprehensive guide on how to use the Eppendorf Micromanipulator 5171 Manual and what benefits it can bring to your research.
Eppendorf Micromanipulator 5171 Manual
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What is the Eppendorf Micromanipulator 5171?
The Eppendorf Micromanipulator 5171 is a device that allows you to control the movement of a microcapillary with high accuracy and sensitivity. It is especially suitable for applications involving large numbers of cells, such as microinjection of plasmids, antibodies, dyes, or nuclear transfection. The device consists of a module unit that can be mounted directly onto the microscope stage, and a separate control board that prevents oscillation and vibration. The module unit has a joystick key that activates the injection function, and other keys that allow you to adjust the speed, angle, limit, and axial mode of the microcapillary. The control board has a display that shows the current settings and status of the device.
How to Connect the Eppendorf Micromanipulator 5171 to Other Devices?
The Eppendorf Micromanipulator 5171 can be connected to other devices that enhance its performance and functionality. For example, you can connect it to the Transjector 5246, which is a device that controls the injection pressure and volume. By doing so, you can achieve semi-automatic injection, where the injection movement and pressure are activated simultaneously at the push of a button. You can also connect it to a CCD camera, which allows you to view and record your microinjection process on a monitor or computer. The Eppendorf Micromanipulator 5171 comes with an interface cable that enables you to connect it to these devices easily.
How to Use the Eppendorf Micromanipulator 5171 Manual?
The Eppendorf Micromanipulator 5171 Manual is a document that provides detailed instructions on how to operate and maintain the device. It also contains important information on safety precautions, troubleshooting tips, technical specifications, and warranty terms. You can access the manual online or download it as a PDF file from the Eppendorf website. To fully understand the key functions and features of the device, it is necessary to read the manual beforehand. The manual also provides diagrams and illustrations that help you visualize the steps and procedures involved in using the device.
What are the Benefits of Using the Eppendorf Micromanipulator 5171?
The Eppendorf Micromanipulator 5171 offers several benefits that make it an ideal device for microinjection and cell manipulation. Some of these benefits are:
It has a high precision and sensitivity that allows you to insert and remove the microcapillary axially into and out of the cell without damaging it.
It has a clear and easy-to-follow keypad that ensures that operating errors are kept to a minimum.
It has a microelectronics and software that guarantee that operating procedures are as "cell-friendly" as possible.
It can be attached to all commonly-used microscopes with the aid of an appropriate adapter.
It can be fixed onto the module unit at any angle and in any direction.
It allows you to determine your own work levels, which minimizes damage to the capillary.
In conclusion, the Eppendorf Micromanipulator 5171 is a device that can help you perform microinjection and cell manipulation with ease and precision. It has optimal functionality and simple operation that make it ideal for applications involving large numbers of cells. By using the Eppendorf Micromanipulator 5171 Manual, you can learn how to operate and maintain the device properly and safely. If you are interested in purchasing or learning more about this device, you can visit the Eppendorf website or contact their customer service.
How to Maintain the Eppendorf Micromanipulator 5171?
The Eppendorf Micromanipulator 5171 is a durable and reliable device that requires minimal maintenance. However, to ensure its optimal performance and longevity, you should follow some basic guidelines on how to clean and store the device. Here are some tips on how to maintain the Eppendorf Micromanipulator 5171:
Clean the device regularly with a soft cloth and a mild detergent. Do not use abrasive or corrosive substances that might damage the device.
Remove any dust or dirt from the module unit and the control board with a soft brush or compressed air.
Check the cables and connectors for any signs of wear or damage. Replace them if necessary.
Store the device in a dry and cool place, away from direct sunlight, heat sources, or magnetic fields.
Do not expose the device to extreme temperatures, humidity, or shocks.
How to Troubleshoot the Eppendorf Micromanipulator 5171?
The Eppendorf Micromanipulator 5171 is a user-friendly and error-free device that rarely encounters any problems. However, if you do experience any issues or malfunctions with the device, you can try some simple troubleshooting steps to resolve them. Here are some common problems and solutions for the Eppendorf Micromanipulator 5171:
ProblemSolution
The device does not turn on or respond.Check the power supply and make sure it is connected properly. Check the fuse and replace it if necessary. Check the interface cable and make sure it is connected properly.
The device does not move or moves erratically.Check the joystick key and make sure it is not stuck or damaged. Check the speed mode and make sure it is appropriate for your application. Check the limit function and make sure it is not activated accidentally. Check the axial mode and make sure it is not activated accidentally.
The device does not inject or injects too much or too little.Check the injection pressure and volume settings and make sure they are appropriate for your application. Check the Transjector 5246 and make sure it is connected properly. Check the microcapillary and make sure it is not clogged or broken.
The device displays an error message or code.Refer to the Eppendorf Micromanipulator 5171 Manual for the meaning and solution of the error message or code. If you cannot resolve the problem yourself, contact Eppendorf customer service for assistance.
In conclusion, the Eppendorf Micromanipulator 5171 is a device that can help you perform microinjection and cell manipulation with ease and precision. It has optimal functionality and simple operation that make it ideal for applications involving large numbers of cells. By using the Eppendorf Micromanipulator 5171 Manual, you can learn how to operate, maintain, and troubleshoot the device properly and safely. If you are interested in purchasing or learning more about this device, you can visit the Eppendorf website or contact their customer service.
How to Choose the Right Microcapillary for the Eppendorf Micromanipulator 5171?
The Eppendorf Micromanipulator 5171 allows you to use different types of microcapillaries for different applications. However, choosing the right microcapillary is crucial for achieving successful and accurate microinjection and cell manipulation. Here are some factors that you should consider when choosing a microcapillary for the Eppendorf Micromanipulator 5171:
The size and shape of the microcapillary tip should match the size and shape of the cell that you want to inject or manipulate. For example, if you want to inject a large cell, you should use a microcapillary with a large and blunt tip. If you want to inject a small cell, you should use a microcapillary with a small and sharp tip.
The material and quality of the microcapillary should be compatible with the solution that you want to inject or manipulate. For example, if you want to inject a solution that contains proteins or DNA, you should use a microcapillary that is made of glass or quartz. If you want to inject a solution that contains salts or acids, you should use a microcapillary that is made of plastic or metal.
The length and diameter of the microcapillary should be suitable for your microscope and micromanipulator settings. For example, if you want to use a high magnification objective, you should use a microcapillary that is short and thin. If you want to use a low magnification objective, you should use a microcapillary that is long and thick.
The Eppendorf Micromanipulator 5171 can accommodate microcapillaries with diameters ranging from 0.5 mm to 2 mm. You can fix the microcapillary onto the module unit at any angle and in any direction. You can also adjust the angle of the microcapillary with the AXIAL key on the control board.
How to Calibrate the Eppendorf Micromanipulator 5171?
The Eppendorf Micromanipulator 5171 is a device that requires periodic calibration to ensure its accuracy and precision. Calibration is a process that involves adjusting the device settings according to a standard or reference point. You should calibrate the Eppendorf Micromanipulator 5171 before using it for the first time, after changing the microcapillary, after changing the microscope objective, or whenever you notice any deviation or inconsistency in the device performance. Here are some steps on how to calibrate the Eppendorf Micromanipulator 5171:
Turn on the device and connect it to the power supply and other devices.
Select the appropriate speed mode and limit function for your application.
Mount the module unit onto the microscope stage and fix the microcapillary onto the module unit.
Focus on your target cell or object with the microscope objective.
Move the module unit with the joystick key until the tip of the microcapillary is aligned with your target cell or object.
Press the HOME/CLEAN key on the control board to exit the work area and store the exit point.
Press the AXIAL key on the control board to switch on the axial mode.
Use the cursor keys on the control board to adjust the angle of the microcapillary until it is perpendicular to your target cell or object.
Press the AXIAL key again to switch off the axial mode.
Press the HOME/CLEAN key again to return to your target cell or object.
Check if your device settings are correct and if your device performance is satisfactory.
If you have any questions or difficulties in calibrating your device, you can refer to the Eppendorf Micromanipulator 5171 Manual for more details and guidance. You can also contact Eppendorf customer service for assistance.
Conclusion
The Eppendorf Micromanipulator 5171 Manual is a valuable resource that can help you use the device effectively and efficiently. The device is a versatile and user-friendly tool that can help you perform microinjection and cell manipulation with ease and precision. It has optimal functionality and simple operation that make it ideal for applications involving large numbers of cells. By reading and following the manual, you can learn how to operate, maintain, troubleshoot, and calibrate the device properly and safely. You can also learn how to choose the right microcapillary, connect the device to other devices, and use the device features and functions. If you are interested in purchasing or learning more about this device, you can visit the Eppendorf website or contact their customer service. 4e3182286b
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